Cells can also be temperature, nanoscale thermometer have unique skills

Recently, a researcher at the Chinese Academy of Sciences institute of genetics and developmental biology strong rainfall team led by cooperating with Peking University, has developed a can give cell quantity & other; Temperature & throughout; Nanoscale thermometer. The nanoscale thermometer by sulfur cadmium selenide CdSeS quantum dots nanostructured luminescent materials and polymer materials, the size is only 30 nanometers, equivalent to one over two thousand five hundred of a human hair, can easily enter the individual cells. Cell is the basic unit of organism structure and function, the temperature of the cell and all sorts of biochemical and physiological processes are closely related. Accurate measurement of the temperature inside the cell will not only help deepen the understanding of cell metabolic activity regularity, but also has potential clinical application value. But the cell size in micron, so tiny life dimension & other; Temperature & throughout; , no doubt, with a smaller thermometer. By detecting light of nanoscale thermometer can & other; Decoding & throughout; The temperature of the corresponding cell. This new type of thermometer is sensitive to temperature, high detection sensitivity, and both the biocompatibility, small impact on the cell itself, more important is the complex biochemical environment can effectively eliminate the cell ( Such as pH, ionic strength, etc. ) On the influence of the temperature detection. Glow of nanoscale thermometer in addition to the influence of temperature, often also is affected by biochemical surroundings; And biochemical environment inside living cells is very complex, moments are in a highly dynamic change, if can't effectively shield around the influence of biochemical environment, will seriously affect the nanoscale thermometer for temperature detection accuracy and reliability in the cell. The team on the basis of past experience building nanostructured luminescent probes, exploration, and constantly practice repeatedly, finally developed a new nano embedding technology, quantum dot encapsulation in a nanometer ball, does not affect the sensitivity of the temperature detection, and effectively eliminate the influence of biochemical surroundings. This new type of nanoscale thermometer, temperature detection sensitivity reached 0. 43℃； Within the scope of the pH value of 4 to 11, its luminous intensity change is less than 2%; When the ionic strength of solution for 500 mM ( Far higher than the physiological condition ~ 100 mM) Only 2%, the luminous intensity change. In addition, this new type of nanoscale thermometer has good repeatability, temperature variations between 25 ℃ and 37 ℃ 100 times, its measurement error is less than 5%. Based on this kind of new nanoscale thermometer, researchers realized the single live temperature changes within the tumor cells, the accuracy and sensitivity of detection; In the single cell level, cells were observed to environment temperature change adaptive thermogenesis. The study in cancer diagnosis and treatment, single-celled drug screening, brain science, and many fields such as electronic micro processing with potential application value. The results have been published online in the science report ( 科学报告) And applied for national invention patent.